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biotinylated horse antimouse igg secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated horse antimouse igg secondary antibody
    Biotinylated Horse Antimouse Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/horse+antimouse+igg+antibodies/R%2ET%2EU%2E+Biotinylated+Horse+Anti-Mouse+IgG+Antibody+(Ready-to-Use)/pm41490873-233-20-29
    Average 93 stars, based on 28 article reviews
    biotinylated horse antimouse igg secondary antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture.
    Article Snippet: .. CSCs cultured in the dynamic cell culture system for 30 days with 0 or 0.1 μM RA onto PA-coated or noncoated coverslips were fixed in 4% paraformaldehyde for 30 min, washed thrice with PBS for 5 min, blocked for 1 h in PBS supplemented with 2% BSA, and incubated with antikeratocan and anti-αSMA primary antibodies (sc-66941, Santa Cruz Biotechnology, Dallas, TX; VPS281, Vector Laboratories) in blocking solution (1:1000) for 2 h; washed thrice with PBS for 5 min and incubated with 1:1000 fluorescein-labeled goat antirabbit and Texas Red-labeled horse antimouse IgG antibodies (Vector Laboratories) and DAPI (Thermo Scientific), respectively, for 1 h. Cells were washed three times with PBS for 5 min and imaged using an A1R confocal laser microscope (Nikon) with constant illumination and capture parameters. .. Micrographs were analyzed using the NIS-Elements and ImageJ v1.46 software packages.

    Incubation:

    Article Title: Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture.
    Article Snippet: .. CSCs cultured in the dynamic cell culture system for 30 days with 0 or 0.1 μM RA onto PA-coated or noncoated coverslips were fixed in 4% paraformaldehyde for 30 min, washed thrice with PBS for 5 min, blocked for 1 h in PBS supplemented with 2% BSA, and incubated with antikeratocan and anti-αSMA primary antibodies (sc-66941, Santa Cruz Biotechnology, Dallas, TX; VPS281, Vector Laboratories) in blocking solution (1:1000) for 2 h; washed thrice with PBS for 5 min and incubated with 1:1000 fluorescein-labeled goat antirabbit and Texas Red-labeled horse antimouse IgG antibodies (Vector Laboratories) and DAPI (Thermo Scientific), respectively, for 1 h. Cells were washed three times with PBS for 5 min and imaged using an A1R confocal laser microscope (Nikon) with constant illumination and capture parameters. .. Micrographs were analyzed using the NIS-Elements and ImageJ v1.46 software packages.

    Blocking Assay:

    Article Title: Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture.
    Article Snippet: .. CSCs cultured in the dynamic cell culture system for 30 days with 0 or 0.1 μM RA onto PA-coated or noncoated coverslips were fixed in 4% paraformaldehyde for 30 min, washed thrice with PBS for 5 min, blocked for 1 h in PBS supplemented with 2% BSA, and incubated with antikeratocan and anti-αSMA primary antibodies (sc-66941, Santa Cruz Biotechnology, Dallas, TX; VPS281, Vector Laboratories) in blocking solution (1:1000) for 2 h; washed thrice with PBS for 5 min and incubated with 1:1000 fluorescein-labeled goat antirabbit and Texas Red-labeled horse antimouse IgG antibodies (Vector Laboratories) and DAPI (Thermo Scientific), respectively, for 1 h. Cells were washed three times with PBS for 5 min and imaged using an A1R confocal laser microscope (Nikon) with constant illumination and capture parameters. .. Micrographs were analyzed using the NIS-Elements and ImageJ v1.46 software packages.

    Microscopy:

    Article Title: Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture.
    Article Snippet: .. CSCs cultured in the dynamic cell culture system for 30 days with 0 or 0.1 μM RA onto PA-coated or noncoated coverslips were fixed in 4% paraformaldehyde for 30 min, washed thrice with PBS for 5 min, blocked for 1 h in PBS supplemented with 2% BSA, and incubated with antikeratocan and anti-αSMA primary antibodies (sc-66941, Santa Cruz Biotechnology, Dallas, TX; VPS281, Vector Laboratories) in blocking solution (1:1000) for 2 h; washed thrice with PBS for 5 min and incubated with 1:1000 fluorescein-labeled goat antirabbit and Texas Red-labeled horse antimouse IgG antibodies (Vector Laboratories) and DAPI (Thermo Scientific), respectively, for 1 h. Cells were washed three times with PBS for 5 min and imaged using an A1R confocal laser microscope (Nikon) with constant illumination and capture parameters. .. Micrographs were analyzed using the NIS-Elements and ImageJ v1.46 software packages.



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